Cellular and Anatomical Pathology Test List

Cellular and Anatomical Pathology Test List
Dept Requested Test Container TAT Special Notes
Cytopathology Ascitic Fluid 50-100 mls maximum required in clean sterile container

5 days*

*>50% reported within 5 days - more complex cases may take longer.

Please avoid sending ascitic fluid in containers larger than 100ml due to storage capacity in cytology department

 

Ascitic fluid should be collected into a sterile container with no fixative solution.

 

It should be submitted to the cytology department as quickly as possible to avoid deterioration.

 

In case of delay the specimen should be stored in a fridge.

Mortuary Autopsy   Up to 3 months dependant on the material retained Consent required. See Anatomical Pathology (Mortuary section)/Requesting a consented autopsy.
Cytopathology Bile Duct Brushings 75-100 mls maximum required in clean sterile container

5 days*

*>50% reported within 5 days - more complex cases may take longer.

Bile duct brushings should be collected into at least 20ml of PreservCyt solution.

 

The specimen container MUST be labelled with a PreservCyt label.

 

PreservCyt fixative solution and labels can be obtained from the cytology department (#38430).

 

Guidance for collection of brushings into PreservCyt solution:

  1. The brush should be placed into a vial of PreservCyt fixative solution
  2. The lid must be secured
  3. The vial must be vigorously agitated
  4. The brush must be removed from the container and disposed of before sending to cytology
Cytopathology Breast Cyst Fluid 75-100 mls maximum required in clean sterile container

5 days*

*>50% reported within 5 days - more complex cases may take longer.

For suspected ALCL – please record this suspicion clearly on the request form to alert staff.

 

Breast cyst fluid should be collected into a sterile container with no fixative solution.

 

It should be submitted to the cytology department as quickly as possible to avoid deterioration.

 

In case of delay the specimen should be stored in a fridge.
Cytopathology Bronchial Brushings

Sterile container containing at least 20mls PreservCyt

5 days*

*>50% reported within 5 days - more complex cases may take longer.

Bronchial brushings should be collected into at least 20ml of PreservCyt solution.

 

The specimen container MUST be labelled with a PreservCyt label.

 

PreservCyt fixative solution and labels can be obtained from the cytology department (#38430).

 

Guidance for collection of brushings into PreservCyt solution:

  1. The brush should be placed into a vial of PreservCyt fixative solution
  2. The lid must be secured
  3. The vial must be vigorously agitated
The brush must be removed from the container and disposed of before sending to cytology.
Cytopathology Bronchial Lavage Sterile container containing 20mls PreservCyt

5 days*

*>50% reported within 5 days - more complex cases may take longer.

 

Bronchial lavage specimens must be collected into at least 20 ml of PreservCyt solution.

 

Containers must be labelled with a PreservCyt label.

 

PreservCyt fixative solution and labels can be obtained from the cytology department (#38430).
Cytopathology Cervical Cytology ThinPrep LBC container

Severn Pathology Laboratory (Bristol)

01686 611333
Cytopathology CSF - Malignant cells 25ml white top universal  

5 days*

*>50% reported within 5 days - more complex cases may take longer.

Arrange with lab prior to sending on ext 38430.  Contact on-call BMS via the Hospital Switchboard if an out of hours request. Reference should be made to special notes in the Cytopathology section.  If sending CSF to more than one department, please send adequate amounts of fluid and seperate request forms appropriate to the tests required.
Cytopathology Cyst Fluids 75-100 mls maximum required in clean sterile container

5 days*

*>50% reported within 5 days - more complex cases may take longer.

Cyst fluid should be collected into a sterile container with no fixative solution.

 

It should be submitted to the cytology department as quickly as possible to avoid deterioration.

 

In case of delay the specimen should be stored in a fridge
Cytopathology EBUS Formalin

5 days*

*>50% reported within 5 days - more complex cases may take longer.

EBUS samples should be collected into 45ml of formalin.

 

Do not refrigerate samples in event of delay
Cytopathology FNA samples

Plastic slide carrier and/or

Sterile container containing at least 30 mls PreservCyt (obtainable from Cytology Department 38430) - brush agitated and removed

and/or

Saline solution in 25 ml conical tube

5 days*

*>50% reported within 5 days - more complex cases may take longer.

Reference should be made to special notes in the Cytopathology section.
Cytopathology Nipple Discharge Plastic slide carrier.

5 days*

*>50% reported within 5 days - more complex cases may take longer.

Each individual slide and the slide container must be labelled with at least 3 points of patient ID.

 

Slides must be allowed to fully air dry before being placed into the container.

Cytopathology Oesophageal Brushings

Plastic slide carrier and/or

Sterile container containing at least 20 mls PreservCyt - brush agitated and removed

5 days*

*>50% reported within 5 days - more complex cases may take longer.

Brushings must be collected into at least 20ml of PreservCyt solution.

 

The container must be labelled with a PreservCyt label.

 

PreservCyt fixative and labels can be obtained from the cytology department (#38430).

 

Guidance for collection of brushings into PreservCyt solution:

  1. The brush should be placed into a vial of PreservCyt fixative solution
  2. The lid must be secured
  3. The vial must be vigorously agitated
  4. The brush must be removed from the container and disposed of before sending to cytology
Cytopathology Pericardial Fluid 50-100 mls maximum required in clean sterile container

5 days*

*>50% reported within 5 days - more complex cases may take longer.

If possible, please submit at least 50ml and a maximum of 100ml.

 

Pericardial fluid should be collected into a sterile container with no fixative solution.

 

It should be submitted to the cytology department as quickly as possible to avoid deterioration.

 

In case of delay the specimen should be stored in a fridge.
Cytopathology Peritoneal Fluid 50-100 mls maximum required in clean sterile container

5 days*

*>50% reported within 5 days - more complex cases may take longer.

If possible, please submit at least 50ml and a maximum of 100ml.

 

Peritoneal fluid should be collected into a sterile container with no fixative solution.

 

It should be submitted to the cytology department as quickly as possible to avoid deterioration.

 

In case of delay the specimen should be stored in a fridge
Cytopathology Pleural Fluid 50-100 mls maximum required in clean sterile container

5 days*

*>50% reported within 5 days - more complex cases may take longer.

If possible, please submit at least 50ml and a maximum of 100ml.

 

Pleural fluid should be collected into a sterile container with no fixative solution.

 

It should be submitted to the cytology department as quickly as possible to avoid deterioration.

 

In case of delay the specimen should be stored in a fridge.

Cytopathology Sputum Wide Mouth containing at least 30 mls PreservCyt (obtainable from Cytology Department 38430)

5 days*

*>50% reported within 5 days - more complex cases may take longer.

Sputum specimens must be collected into at least 20 ml of PreservCyt solution.

 

Containers must be labelled with a PreservCyt label.

 

PreservCyt fixative solution and labels can be obtained from the cytology department (#38430).

 

To increase sensitivity of test:

  1. Sputum should be obtained from a deep cough
  2. Sputum samples should be submitted each day for three consecutive days (do not pool samples)
Cytopathology Urine 75-100 mls maximum required in clean sterile container

5 days*

*>50% reported within 5 days - more complex cases may take longer.

Please avoid submitting urine from the first urination of the day.

 

Please submit the whole sample if possible.

 

Do not collect mid-stream urine only.

 

Urine should be collected into a sterile container with no fixative solution.

 

It should be submitted to the cytology department as quickly as possible to avoid deterioration.

 

In case of delay the specimen should be stored in a fridge.

Histopathology Copper (Dry weight) Dry container 6 weeks Annotate 'DRY COPPER WEIGHT REQUEST' on request form.  Place specimen dry in wide mouth container.  DO NOT ADD FORMALIN. Specimen sent to another centre for testing.
Histopathology Electron Microscopy 4.0% gluteraldehyde 1 month Arrange with the Histology laboratory prior to taking sample- tel extn 52362
Histopathology Frozen Section Send sample in dry pot

Less than 1 hour *complex/multiple blocks/multiple specimens may take longer.

Arrange with Histology Laboratory prior to taking sample - tel extn 52362 . See notes in 'Sending High Risk Specimens' section if patient is known, or suspected, to be an infection hazard.*Please note that we are no longer able to offer a frozen section service to count neutrophils in specimens from orthopaedic joint revision surgery (see explanatory note in Histopathology Section)
Histopathology Histological examination Formalin unless frozen section or other specialist request 3-14 days Most specimens are reported within the 3-14 day period.  Complex or specimens that need more consideration or additional special investigations will take longer to report.  
Histopathology Immunofluorescence (IMF) - skin/mucosal biopsies  In time for the next Dermatology CPC Meeting (following the date of the biopsy) In time for the next Dermatology CPC Meeting (following the date of the biopsy) Reference should be made to special notes in 'Immunofluorescence (IMF) on skin and mucosal biopsies'  section
Histopathology Iron (Dry weight) Dry container 6 weeks Annotate 'DRY IRON WEIGHT REQUEST' on request form.  Place specimen dry in wide mouth container.  DO NOT ADD FORMALIN. Specimen sent away for testing.
Histopathology Ovarian tumours Dry container 3-14 days Reference should be made to special notes in the 'Gynaecological (ovarian and endometrial tumours)' section. Most specimens are reported within the 3-14 day period.  Complex or specimens that need more consideration or additional special investigations will take longer to report.  
Histopathology Renal Biopsy Provided by BMS on attendance at the biopsy. Alternatively biopsies may be transported direct to the lab in saline soaked gauze for examination and transfer to the correct containers. 3-14 days BMS attendance at renal biopsies is by arrangement. Telephone the laboratory 52362. A BMS will attend if possible; this may be difficult over the lunchtime period 12.30 – 13.30. (Biopsies may be transported direct to the laboratory for examination, in cases of emergency). Biopsies must be received before 16.00 to ensure processing overnight.  
Histopathology Skin for Immunofluorescence (IMF)   3 weeks Reference should be made to special notes in 'Immunofluorescence (IMF) on skin and mucosal biopsies'  section
Neuropathology Intra-operative diagnosis -Frozen section/brain smear Send sample in dry pot Less than 1 hour Arrange with laboratory prior to taking sample - tel extn 54408 . See notes in 'Sending High Risk Specimens' section if patient is known, or suspected, to be an infection hazard.
Neuropathology Muscle Biopsies   2 weeks Reference should be made to special notes in the 'Muscle and Nerve Biopsies' section.
Neuropathology Nerve Biopsies   4-6 weeks Reference should be made to special notes in the 'Muscle and Nerve Biopsies' section.
Mortuary Post Mortem Examination   Up to 3 months dependant on the material retained Consent required. See Anatomical Pathology (Mortuary section)/Requesting a consented autopsy.
Neuropathology Skin biopsy for small fibre neuropathy   2 weeks Arrange with lab prior to sending on ext 54408 and see special notes in 'Skin biopsies for Intraepidermal Nerve Fibre Density' section.
Neuropathology/ Molecular Biology MGMT assay     Contact either Dr D Hilton on 31360 or Dr A Shivane on 32033
Neuropathology/ Molecular Biology Loss of hetrozygosity 1p and 19q in oligodendrogliomas 10ml EDTA blood   Contact Joanna Farrugia, Molecular biology 52408.


 

Review Status

Reviewed December 2022
This page will be updated June 2023

Was this page helpful?

Was this page helpful?
Rating

Please answer the question below, this helps us to reduce the number of spam emails that we receive so that we can spend more time responding to genuine enquiries and feedback. Thank you.

*